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KMID : 0861020180330030019
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Korea Journal of Herbology
2018 Volume.33 No. 3 p.19 ~ p.24
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Anti-inflammatory Effect of the Hedgehog Skin Extracts on LPS-Stimulated RAW 264.7 Cells
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Kim Tae-Yeon
Jo Na-Young
Leem Kang-Hyun
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Abstract
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Objectives : Hedgehog skin is one of the animal medicines in Traditional Korean Medicine for hematochezia and hemorrhoids. In this study, we examined cytotoxicity and anti-inflammatory effects.
Methods : Cytotoxicity of hedgehog skin extracts was measured by MTT assay in vitro. We investigated the inhibition of lipopolysaccharide (LPS) stimulated nitric oxide (NO) production in RAW 264.7 cells. The phosphorylation of mitogen-activated protein kinases (MAPKs) was measured by western blot. And we observed the effect of hedgehog skin extracts on the expression of IL-6 genes using real time PCR.
Results : As a result of MTT assay for cytotoxicity, there were no significant differences between non-treatment group and hedgehog skin extracts treatment groups. 500 ㎍/㎖ of hedgehog skin extracts treatment significantly decreased nitric oxide production in comparison with non-treatment in LPS-induced RAW 264.7 cells. In measurement of the phosphorylation of MAPKs using western blot analysis, LPS stimulation increased the phosphorylation of MAPKs and 500 ㎍/㎖ of hedgehog skin extracts treatment decreased the phosphorylation of ERK1, ERK2 and p38 significantly. But there were no significant differences the phosphorylation of JNK1 and JNK2. As a result of confirmation of the IL-6 mRNA gene expression using real time PCR, IL-6 mRNA gene expressions were significantly decreased in 50 ㎍/㎖, 100 ㎍/㎖ and 500 ㎍/㎖ hedgehog skin extracts treated groups by comparison with non-treatment group.
Conclusion : These results could provide a mechanistic explanation for the anti-inflammatory effects of the hedgehog skin.
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KEYWORD
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Hedgehog skin, Anti-inflammatory, Hemorrhoids, Mitogen-activated protein kinases (MAPKs), IL-6
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